产品货号：
GS4629
中文名称：
重组人ME2(带His标签)
英文名称：
Recombinant Human Malic Enzyme 2, His
产品规格：
5μg
发货周期：
1～3天
产品价格：
询价
分子量：Approximately 64.4kDa,a single non-glycosylated polypeptide chain containing 573 amino acids with 6×His at C-terminus.
储存条件：冷冻（-20℃）
概述
NAD-dependent malic enzyme(ME2),mitochondrial is a protein that in humans is encoded by the ME2 gene.This gene encodes a mitochondrial NAD-dependent malic enzyme,a homotetrameric protein,which catalyzes the oxidative decarboxylation of malate to pyruvate.Three different isoforms of ME are known to be in mammalian tissues:a strictly cytosolic NADP+-dependent enzyme,an NADP+-dependent mitochondriail isoform,and a mitochondrial isoenzyme that is able to use both NAD+ and NADP+ but is more effective with NAD+.The mammalian isoforms size is about 62-64 kDa.A native size of 240,000 Da proposes a tetrameric structure for the active enzyme.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
相关搜索：重组人ME2(带His标签)，ME2, His-tagged, Human，Recombinant Human Malic Enzyme 2, His
储存条件：冷冻（-20℃）
概述
NAD-dependent malic enzyme(ME2),mitochondrial is a protein that in humans is encoded by the ME2 gene.This gene encodes a mitochondrial NAD-dependent malic enzyme,a homotetrameric protein,which catalyzes the oxidative decarboxylation of malate to pyruvate.Three different isoforms of ME are known to be in mammalian tissues:a strictly cytosolic NADP+-dependent enzyme,an NADP+-dependent mitochondriail isoform,and a mitochondrial isoenzyme that is able to use both NAD+ and NADP+ but is more effective with NAD+.The mammalian isoforms size is about 62-64 kDa.A native size of 240,000 Da proposes a tetrameric structure for the active enzyme.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
| 外观 | Sterile Filtered White lyophilized(freeze-dried)powder. |
| 纯度 | ＞95%by SDS-PAGE and HPLC analyses. |
| 来源 | Escherichia coli. |
| 生物活性 | Malic Enzyme activity was assayed spectrophotometrically at 340nmas described in Mandela and Sauer(1975).The standard reaction mixture contained 50mMTris-HCl,3 mM MnCl2,5 mM malate,0.12 mM NADP+,2.5 mM fumarate.Assay was performed in a Beckman spectrophotometer.The Km value is 1.5 ± 0.6 mM. |
| 成分 | Lyophilized from a 0.2μmfiltered concentrated solution in PBS,pH 7.4. |
| 内毒素 | Less than 1 EU/μg of rHuME2,His as determined by LAL method. |
| 复溶 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃.Further dilutions should be made in appropriate buffered solutions. |
| 氨基酸序列 | MLHIKEKGKP LMLNPRTNKG MAFTLQERQM LGLQGLLPPK IETQDIQALR FHRNLKKMTS PLEKYIYIMG IQERNEKLFY RILQDDIESL MPIVYTPTVG LACSQYGHIF RRPKGLFISI SDRGHVRSIV DNWPENHVKA VVVTDGERIL GLGDLGVYGM GIPVGKLCLY TACAGIRPDR CLPVCIDVGT DNIALLKDPF YMGLYQKRDR TQQYDDLIDE FMKAITDRYG RN |
相关搜索：重组人ME2(带His标签)，ME2, His-tagged, Human，Recombinant Human Malic Enzyme 2, His